We then calculated the co-localization ratio between loop anchor and H3K27ac modification or CTCF binding sites and found that T-ALL-specific loop anchors exhibit a significant enrichment of T-ALL-specific H3K27ac modification or CTCF binding, the same pattern was also seen in normal T cells (Supplementary Fig. 1g). The gene discussed is CTCF; the disease is acute lymphoblastic leukemia.