We further cocultured mouse splenic T cells with Hepa1–6–WT, Hepa1–6-Nectin2–KO-1 (KO1), Hepa1–6-Nectin2–KO-2 (KO2), or Hepa1–6–Nectin2–KO-3 (KO3) stable HCC cells. This evidence concerns the gene NECTIN2 and hepatocellular carcinoma.