However, to test this in our model, mouse melanoma cell lines were established and characterized from the different independent Braf-Pten-Brn2 C57BL/6 J mouse melanoma; the m50 and m6 cell lines were Brn2-WT, m59, and m36 were Brn2-het, and m82 and m8 were Brn2-hom (Supplementary Fig. 4A–F). The gene discussed is PTEN; the disease is melanoma.