POU3F2 and melanoma: Although these observations are consistent with BRN2 affecting human melanoma initiation and progression, loss of the BRN2 locus is frequently associated with large segmental deletions that affect the long arm of chromosome 6 (6q) as it was observed in 14 out of 53, and 9 out of 32 out of primary melanoma and in 17 out of 21 melanoma cell lines52–54, and confirmed in this study on a total of 205 out of 338 melanomas, and 11 out of 25 melanoma cell lines.