AKT1 and Huntington disease: Consistent with RNA-seq results, a lower level of p-AKT was observed in PT MФs compared to that in GGF MФs by western blot analysis (Fig. 3i).To further confirm whether the PI3K-AKT pathway potentially upregulated in M2 MФs, M1 MФs, and M2 MФs from HD BM were investigated using RNA-seq (Fig. 3j).