As shown in Figures S5C, D, we observed that mice injected with ABL-depleted MDA-MB231 cells had a smaller number of lung metastases than those in mice injected with control cells, suggesting that ABL-mediated RUNX2 expression and activity regulate the invasive capacity as well as seeding and growth of breast cancer cells in lung metastasis. This evidence concerns the gene ABL1 and breast cancer.