We found positive staining of tumor cells in IHC staining for VISTA in 2.1% (3/145), Galectin-9 in 36.6% (53/145), PD-L1 in 40.7% (59/145), CEACAM-1 in 57.2% (83/145), HVEM in 74.5% (108/145), PVR in 77.9% (113/145) and HLA-E in 84.8% (123/145) of patients (Fig. 3c)29–33. This evidence concerns the gene LGALS9 and neoplasm.