To identify the relationship between DNA methylation and AK5 expression, we performed qMSP and real-time PCR in one normal colon cell sample (CCD18Co), six colon cancer cell samples (HT-29, SW480, DLD-1, Lovo, HCT116, KM12SM), and two rectal cancer cell samples (SNU-70, SNU-796). This evidence concerns the gene AK5 and rectal cancer.