Univariate analysis did not show a significant correlation between AK5 expression and survival, but poor differentiation (P = 0.015), larger tumour size (P = 0.003), higher T stage (P = 0.004), LVI (P < 0.001), PNI (P < 0.001), LN metastasis (P < 0.001), positive resection margin (P < 0.001), and DM (P < 0.001) were correlated with poor prognosis. The gene discussed is AK5; the disease is neoplasm.