TNF and type 2 diabetes mellitus: Without any RvE1 treatment, we observed higher levels of pro-inflammatory cytokines P-Selectin and TNF-α among T2D compared to healthy neutrophils, consistent with recent evidence for enriched cytokine expression in T2D neutrophils [44], though levels of pro-inflammatory MIP-1β, IL-8, and sICAM-1 cytokines were decreased in T2D neutrophils (Fig. 4).