In this study, mouse models of sepsis were establishedin vitro, and the effects of IL-33 on the NF-κB/p38 MAPKsignaling pathway and pyroptosis of macrophages in mice with sepsis were assessed.The results showed that: 1) IL-33 had a close relation to the occurrence anddevelopment of sepsis; 2) IL-33 could induce the pyroptosis of macrophages in micewith sepsis; and 3) IL-33 activated the NF-κB/p38 MAPK signaling pathway toup-regulate the expression of pro-caspase-1 p10, a pyroptosis-related protein inmacrophages, thus mediating the pyroptosis of macrophages in mice with sepsis. The gene discussed is IL33; the disease is Sepsis.