To further curtail AML-associated regions for targeted analysis, we selected four CpGs with perturbation in most AML samples, which were associated with the genes for RHO family interacting cell polarization regulator 2 (FAM65B; cg15289427), piezo type mechanosensitive ion channel component 1 (FAM38A; cg27630153), and protein kinase CAMP-activated catalytic subunit alpha (PRKACA; cg19586199), while cg22797031 is not associated with a specific gene. This evidence concerns the gene PRKACA and acute myeloid leukemia.