Gene-set enrichment analysis revealed the enrichment of PAX3-FOXO1-associated genes in Cluster 1 (GRYDER_PAX3FOXO1_TOP_ENHANCERS, Supplementary Fig. 5c)30, whereas Cluster 2, which included ES associated with EWSR1-FLI1 fusion, undifferentiated sarcoma, and other tumors, was not associated with specific highly expressed genes (Supplementary Fig. 5b). This evidence concerns the gene EWSR1 and undifferentiated (embryonal) sarcoma.