To determine the source of the increased number of autophagosomes mediated byIL-6 treatment, we examined the levels of free GFP (GFP protein freed from the fusion protein GFP-LC3B upon autophagic flux enhancement) and SQSTM1 (p62) expression in cancer cells transfected with a GFP-LC3B plasmid. Here, MAP1LC3B is linked to cancer.