To test whether IFN-I signaling plays a role in inducing neurological disorders (JE) following JEV infection, IFN-I signal-competent (BL/6) and -incompetent (IFNAR1 KO) mice were infected with JEV at different doses (1 × 106, 5 × 106, 1 × 107, and 5 × 107 ffu) via the intraperitoneal or footpad route for systemic or local infection, respectively. This evidence concerns the gene IFNAR1 and Japanese encephalitis.