EGFL8 and hepatocellular carcinoma: Subsequently, we performed a Transwell invasion assay and the results showed that the number of HCCLM3EGFL8 cells invaded passed through the matrigel was much less than that of HCCLM3Vector cells (72.33 ± 3.33 versus 148.70 ± 7.21, P < 0.001; Fig. 2d) but the number of Hep3BshEGFL8 cells invaded passed through the matrigel was more than Hep3BshCtrl cells (105.30 ± 6.98 versus 52.33 ± 4.26, P = 0.0008; Fig. 2d), further suggesting a suppressing role of EGFL8 on the metastasis and invasion of HCC cells.