Furthermore, we treated EGFL8 depleted Hep3B cells with DAPT, an inhibitor of Notch signaling pathway [21, 29], and found this treatment obviously inhibit the expression of Notch signaling pathway and significantly suppressed the migration, invasion, and survival of Hep3B cells which had been remarkably enhanced by EGFL8 knockdown, suggesting the modulation of EGFL8 on liver cancer cell metastasis and apoptosis is, at least partially, depend on the inhibition of Notch signaling pathway. The gene discussed is EGFL8; the disease is liver cancer.