To understand the role of EGFL8 in HCC development, we employed lentivirus-mediated gene transfer or shRNA to enhance or suppress the EGFL8 expression in HCCLM3 or Hep3B cell line, which had the lowest or highest expression level of EGFL8 among the three liver cancer cell lines we tested respectively. This evidence concerns the gene EGFL8 and hepatocellular carcinoma.