NOTCH1 and hepatocellular carcinoma: Additionally, H&E stain confirmed the diagnosis of HCC for the subcutaneous xenograft tumors of two groups of mice and the results of IHC assay showed a significantly increased EGFL8 expression and an obviously decreased Notch1 expression in the tumors of HCCLM3EGFL8 cells compared with those tumors of HCCLM3Vector cells (Fig. 3f).