To verify this hypothesis, we detected the expression levels of Notch signaling pathway including Notch1, NICD, Hes1, and Hey1 in liver cancer cells by Western blot, and the results exhibited a significant decrease of these proteins in EGFL8 overexpressed HCCLM3 cells and an obvious increase of their expression in EGFL8 depleted Hep3B cells. The gene discussed is NOTCH1; the disease is liver cancer.