To verify this hypothesis, we detected the expression levels of Notch signaling pathway including Notch1, NICD, Hes1, and Hey1 in liver cancer cells by Western blot, and the results exhibited a significant decrease of these proteins in EGFL8 overexpressed HCCLM3 cells and an obvious increase of their expression in EGFL8 depleted Hep3B cells. This evidence concerns the gene HEY1 and liver cancer.