Using helical reconstruction in RELION3.1, we determine a 3.2 Å resolution map of the ordered core of PHFs from GSS (F198S) (Fig. 3b, f), with the same crossover distance and width of PHFs from PrP-CAA (Q160X), and the same monomeric fold as the AD fold, i.e., the C-shaped unit (Figure S3). The gene discussed is PRNP; the disease is Alzheimer disease.