In order to evaluate the presence and the proportion of CSCs, ALDH enzymatic activity was assessed using the ALDEFLUOR assay in three lung adenocarcinoma cell lines with different KRAS and EGFR mutational status: two KRAS mutant cell lines (A549 and H1734) and one EGFR mutant cell line H1975. Here, LDHA is linked to lung adenocarcinoma.