CD8A and neoplasm: To evaluate the functionality of p32 murine CAR T cells we used two different p32+ tumor-derived cell lines, one maintained in a differentiated state, AFFR53, and another line, 005 that we have previously characterized as GSC and that forms typical neurospheres (also termed “tumorspheres”)29 (Supplementary Fig. 4a, b and Supplementary Table 1).