For this purpose, we collected 111 clinical cases of stage II–IV colonic tumor tissues and their distal colonic normal tissues and then measured the expression of NCX1, NCX2, and NCX3 by quantitative polymerase chain reaction (qPCR), western blotting (WB), and immunohistochemistry (IHC). The gene discussed is SLC8A1; the disease is colonic neoplasm.