It was reported earlier that N-dimethyl-D-erythro-sphingosine (DMS), an inhibitor of protein kinase C and sphingosine kinase, can induce an increase in the intracellular calcium concentration ([Ca+]i) in HCT116 colon cancer cells, which suggested that NCX was positively involved in DMS-induced [Ca2+]i increase by using NCX inhibitors a priori and NiCl2 to block NCX’s reverse transport of calcium ions into the cell to reduce [Ca2+]i [26]. This evidence concerns the gene TLX2 and colonic neoplasm.