The qRT-PCR result indicated that, compared to the normal HFFs, T. gondii RH and CEP infection led to significantly upregulated transcription of TRIM21 at 1 h post-infection; however, with the infection going on, only T. gondii RH infection kept the significantly upregulated transcription of TRIM21 at 24 h post-infection, CEP infection led to a similar transcription level as in the uninfected cells (Figure 4A). This evidence concerns the gene TRIM21 and infection.