GO functions analysis results showed that the reduced genes in ESCC were related to fatty acid degradation, fatty acid metabolism, Mucin-type O-glycan biosynthesis, N-glycan biosynthesis, peroxisome, Fc gamma R-mediated phagocytosis, metabolic pathways, and endocytosis (Figure 4C). The gene discussed is MUC5AC; the disease is esophageal squamous cell carcinoma.