However, when transfected H9c2 cells with Sp1 shRNA, the expression of PARP1 and autophagy-related proteins decreased, whereas infecting H9c2 cells with AD-PARP1 adenovirus upregulated the expression of PARP1 and autophagy-related proteins again (Figure 5D). The gene discussed is PARP1; the disease is Alzheimer disease.