According to western blot results, the expression of PARP1 and autophagy-related proteins decreased when rats were treated with AG-14361 or transfected with Sp1 shRNA, whereas rats were treated with BEZ235 or infected with Sp1 shRNA and AD-PARP1 adenovirus increased the expression of PARP1 and autophagy-related proteins again (Figure 6E). The gene discussed is SP1; the disease is Alzheimer disease.