Although it was reported by Araki (Araki et al., 2009) that after 30 days of viral challenge and rapamycin treatment there was minimal incorporation of BrdU into DNA of specific CD8+ T-cells in all groups, showing that the decrease in T-cell contraction was not due to increase of cell proliferation but probably by survival, our data indicate that modulation exerted by rapamycin may inhibit cell contraction by different mechanisms, depending on the infection model and vaccine protocol used. Here, CD8A is linked to infection.