The group with high Nanog expression (Nanog>6.7) had a high total number of CTCs (Figure S2, P=0.0001), E-CTCs (Figure 3C, P=0.003), mixed CTCs (Figure 3D, p<0.001), and M-CTCs (Figure 3E, p<0.001).In order to explore whether the various phenotypes of CTC and the expression of CSCS marker (Nanog) are related to the occurrence of liver cancer, we used 160 cases of HCC tissue chip array for immunohistochemical analysis. Here, NANOG is linked to hepatocellular carcinoma.