AFP and hepatocellular carcinoma: Univariate analysis of prognostic factors of HCC has been the following: Tumor size > 5 cm (P =0.049), BCLC stage (B+C) (P = 0.024), AFP > 400 μg/L (P = 0.002), PIVKA > 400 μg/L (P=0.039), Nanog > 6.7 (P = 0.005), and HBV-DNA > 100 (P=0.017).