ART4 and female infertility: Our findings indicated that in the case of fallopian tubal blockage as a cause of female infertility, the factors of both males (sperm volume and viability, etc.)and females (follicular alanine, glutamine, and cytoplasmic status, etc.)need to be evaluated, while in the case of male infertility caused by sperm problems, oocyte quality in terms of the follicular metabolites index (especially glycoprotein, lipids, and acetic acid) as a compensatory role should be considered.