IKBKG and chronic granulomatous disease: There may be some limitations in utilizing commercially available WES as some genes such as IKBKG associated with NEMO deficiency and NCF1 associated with CGD may have pseudo gene loci (60, 73, 74) Whole genome sequencing (WGS) offers the ability to capture further sequencing with the capacity to pick up copy number variations and examine noncoding areas with promoters and enhancers (72, 75, 76).