Moreover, flow cytometry and gene expression analysis of CD4+ T cell subsets revealed that highly infiltrated breast cancers also harbor TLS, and express markers such as Cxcl13, ICOS, IFNγ and TBX21/T-bet, commonly associated with follicular T helper (Tfh) and Th1 profiles (39, 40). This evidence concerns the gene CXCL13 and breast cancer.