Additionally, the number of CAR-T cells and tumor cells, as well as the expression of exhaustion markers (PD-1 and TIM-3) were determined to exhibit the key parameters, including the maximal CD19.CAR-T cell expansion, challenging times, residual tumor cells in the end of coculture, and exhaustion status of CD19.CAR-T cells that could determine the early expansion and persistence of CD19.CAR-T cells. The gene discussed is CD19; the disease is neoplasm.