Seizure induced increase of c-Fos labeled cells in vlPAG both in naïve rats (Pilo group) and chronic epilepsy rats (SE+Pilo group) compared to control rats, but a significant lower increase in SE+Pilo group rats compared to Pilo group rats [Figure 5B, One-way ANOVA for comparison among groups, F(2, 18)= 12.135, p = 0.001; Pilo vs. control, p = 0.000; SE+Pilo vs. control, p = 0.042; SE+Pilo vs. Pilo, p = 0.007]. Here, FOS is linked to Seizure.