TP53 and nicotine dependence: We discovered that upregulated DEGs were significantly enriched in p53 signaling pathway, complement and coagulation cascades, progesterone-mediated oocyte maturation, oocyte meiosis, neuroactive ligand-receptor interaction, and cell cycle, whereas downregulated DEGs were significantly enriched in retrograde endocannabinoid signaling, dopaminergic synapse, nicotine addiction, amyotrophic lateral sclerosis (ALS), synaptic vesicle cycle, and neuroactive ligand-receptor interaction.