To determine the effector capacity of tumor-infiltrating T cells (TILs) from NC410-treated mice, we re-stimulated TILs from digested tumors with anti-CD3 plus anti-CD28 for 5 hr and performed intracellular staining to examine IFN-γ and TNF-α production (Figure 5E). The gene discussed is CD28; the disease is neoplasm.