The aims of this study were to investigate: (i) the effects of AN on CIS-induced neurotoxicity of primary DRG neurons, (ii) the influences of AN on allodynia and hyperalgesia in CIS-induced peripheral neuropathy in rats and (iii) possible ability of nonselective NOS inhibitor lNAME to potentiate the effect of AN. Here, NOS1 is linked to in situ carcinoma.