ABCG8 and steatosis: Due to the similarities between iHLC and developing liver, we used iHLC to study the susceptibility to steatosis that may occur in the immature liver by exposing iHLC to an exogenous plant sterol in setting of decreased ABCG8. We found that iHLC accumulate intracellular lipid in a concentration-dependent manner when exposed to stigmasterol in ranges seen in the plasma of infants receiving TPN with soy lipid17.