Similarly, the KEGG pathway for NSCLC (Supplementary Fig. 10A) and the Ras signaling pathway (Supplementary Fig. 10B), which regulates cell growth and can activate several other signaling pathways like the PI3K-Akt pathway or the Raf/MAPK pathway45, can also be largely covered on a phosphoproteome level by both the equal loading and microscaled TMT approach. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.