HNF4A and hepatocellular carcinoma: An important discovery is that our assay is highly sensitive to the level of activity of the nuclear hormone receptor hepatocyte nuclear factor 4α (HNF4α)8 and the T6PNE cells used for the assay have a low level of stored fat at baseline, unlike hepatoma cell lines, which we find to be highly steatotic, making it possible to more easily study the relationship between HNF4α activity and stored fat.