In order to examine the stemness of these cells, colonies of these cells were excised under a microscope and dispersed by trypsin/EDTA treatment, and the presence of cancer stem cell markers (CD44 [14, 16, 17], CD133 [14, 15, 16, 17], Oct3/4 [14, 18], ALP [16]) was examined in these free cells. This evidence concerns the gene PROM1 and cancer.