Herein, we observed that CXCL2 stimulation of CT-26 cells increased adhesion of colon cancer cells to vitronectin, fibronectin, collagen IV, laminin I and fibrinogen but not to collagen I. These findings were partially recapitulated in human cells showing that CXCL8 increased HT-29 cell adhesion to fibronectin and laminin I. Notably, flow cytometry revealed that both murine and human colon cancer cells express the αV and β3 integrin subunits. Here, CXCL8 is linked to colonic neoplasm.