FLT3 and leukemia: Next-generation sequence analyses were conducted of the leukemia relevant mutations of 22 genes, including FLT3-ITD, NPM1, KIT, CEBPA, DNMT3A, IDH1, IDH2, TET2, EZH2, RUNX1, ASXL1, PHF6, TP53, SF3B1, SRSF2, U2AF1, ZRSR2, NRAS, CBL, SETBP1, ETV6, and JAK2, with the method as described in our previous publication.[12] For the gene length longer than 150 bp, such as FLT3-ITD, alternative RT-PCR method was used for analysis.