The overall median KLF4 expression was of 48 and 195 in the HCC and NAFLD cases, whereas the median of expression value of USP11 was 207 and 986, respectively, as calculated by a normalization method of RNA‐Seq by Expectation‐Maximization (RSEM) software. This evidence concerns the gene KLF4 and metabolic dysfunction-associated steatotic liver disease.