In the present study, we generated Awat1 KO, Awat2 KO, and Awat1 Awat2 double KO (DKO) mice and used them to examine the contribution of Awat1 and Awat2 to the production of meibum lipids, as well as their roles in dry eye prevention. Here, AWAT2 is linked to Keratoconjunctivitis sicca.