Firstly, to determine the differential expression of GCSC markers, the mRNA expression of EPCAM, ICAM1, ALDH1, THY1, PROM1, TFRC, CD24, OCT4, SOX2, LGR5, NANOG, ABCB1, ABCG2, CXCR4, ALCAM, DCLK1, ITGA6, and MSI1 in GC and normal stomach tissues were analyzed using the Oncomine and GEPIA databases, respectively. This evidence concerns the gene POU5F1 and gastric cancer.