Firstly, to determine the differential expression of GCSC markers, the mRNA expression of EPCAM, ICAM1, ALDH1, THY1, PROM1, TFRC, CD24, OCT4, SOX2, LGR5, NANOG, ABCB1, ABCG2, CXCR4, ALCAM, DCLK1, ITGA6, and MSI1 in GC and normal stomach tissues were analyzed using the Oncomine and GEPIA databases, respectively. Here, DCLK1 is linked to gastric cancer.