We also used the GEPIA2 tool to analyze the relationship between TWF1 expression and tumor pathological staging, which indicated stage-specific expressional changes in TWF1 expression in case of a few tumor types, such as HNSC, LIHC and LUAD (Figure 1D, all P<0.05), while in most cases we found no clear association (Figure S5). The gene discussed is TWF1; the disease is neoplasm.