In the present study, we assessed the factors such as age, gender, eyes, clinical history of thyroid disease and TED, type of thyroid disease, antithyroid therapy, degree of exophthalmos, clinical activity, thyroid hormone, antibody levels, UR of extraocular muscles in SPECT/CT, VACI, and the combination of parameters to find an efficient and reliable diagnostic test for DON. This evidence concerns the gene TG and thyroid gland disorder.