Next, we evaluated these ADCs for in vitro cytotoxicity in HER2-positive (KPL-4, JIMT-1, and SKBR-3) and -negative (MDA-MB-231) breast cancer cell lines, human embryonic kidney 293 (HEK293) cells, and human hepatocyte carcinoma (HepG2) cells (Fig. 3 and Supplementary Fig. 2). The gene discussed is ERBB2; the disease is breast carcinoma.