Compared to the parental D2A1 cells, the selected D2A1-m12 subline rapidly forms macrometastatic lesions in the Endo180−/− BALB/c mice following intravenous inoculation (Fig. 8a) and, in contrast to the parental and other mouse mammary carcinoma lines tested (Fig. 2e–i), gives rise to an equivalent metastatic burden when inoculated intravenously into Endo180−/− and wildtype mice (Fig. 8b). This evidence concerns the gene MRC2 and breast carcinoma.