Equivalent results are obtained with D2A1 tumour cells admixed with CAFs at different ratios, D2A1 cells admixed with mouse CAFs transduced with two independent shRNAs targeting Endo180, and 4T1 tumour cells admixed with CAFs or normal fibroblasts (Supplementary Figs. 4b, c and  5a–c). The gene discussed is MRC2; the disease is neoplasm.