Consistent with these findings, 4T1, D2A1, and D2A1-m2 primary tumours in Endo180−/− mice have a significant deficit in intra-tumoural αSMA-positive cells (Fig. 7a), and a reduction in intra-tumoural fibrillar collagen deposition as detected by Masson’s trichrome staining (Fig. 7b). Here, ACTA1 is linked to neoplasm.