MRC2 and neoplasm: After orthotopic inoculation, the D2A1-m2 primary tumours grow equally well in both wildtype and Endo180−/− mice, but there remains a significant defect in their ability to spontaneously metastasise in the Endo180−/− mice (Fig. 2d and Supplementary Fig. 2b) confirming that all three cell lines examined show impaired tumour progression, but that the defects manifested vary between the lines.