RT-qPCR analysis validated the increased expression of selected matrisomal collagens and glycoproteins identified in the D2A1-m12 RNA-Seq data and, conversely, validated the downregulated expression of Bgn and Ltpb2 (Fig. 8f) These findings suggest that the enhanced tumourigenesis of the D2A1-m12 subline in Endo180−/− mice results from tumour cells acquiring an enhanced ability to remodel and respond to the ECM, when CAF viability is impaired. Here, MRC2 is linked to neoplasm.