CD8A and neoplasm: NY-ESO-1-specific human CD8+ T cells were generated by transducing healthy donor peripheral blood CD8+ T cells with a Retro/ESO-TCR retroviral vector encoding a NY-ESO-1-specific TCR (clone 3A1; denoted as ESO-TCR); the resulting T cells, denoted as ESO-T cells, expressed ESO-TCRs and specifically targeted A375-A2-ESO tumor cells, thereby modelling the tumor-specific human CD8+ T cells (Supplementary Fig. 6e, f).