PRF1 and hepatocellular carcinoma: HCC specimens with high Siglec-10hi TAM infiltration exhibited lower proportions of granzyme B+ (GZMB+), IFN-γ+, IL-2+ and perforin-1+ (PRF-1+) CD8+ CTLs (Fig. 3c), but higher proportions of CTLA-4+, LAG-3+, PD-1+, TIGIT+ and TIM-3+ CD8+ CTLs (Fig. 3d) compared to samples with low Siglec-10hi TAM infiltration.