The present study found that the expression of GPC3 in NSCLC is lower than that in paracancer tissues, which is in line with a study by Kim et al. [26] and in contradiction with a study by Sarit et al. In addition, the present study found that GPC3 was not differentially expressed in the smoker vs. non-smoker group or the LUSC smoker vs. LUSC non-smoker group. The gene discussed is GPC3; the disease is non-small cell lung carcinoma.