ACACA and neoplasm: Primers designed for an intrachromosomal 17 junction hitting genes ACACA and PLCD3 (Fig 3Bi) positively detected ctDNA in both pre- and post-surgical plasma cfDNA by qPCR (Ct values 34.797 and 33.841 respectively), with similar amplicon melting profile to primary tumor DNA, and no signal in negative controls (Fig 3Bii).