Primers designed for an intrachromosomal 17 junction hitting genes ACACA and PLCD3 (Fig 3Bi) positively detected ctDNA in both pre- and post-surgical plasma cfDNA by qPCR (Ct values 34.797 and 33.841 respectively), with similar amplicon melting profile to primary tumor DNA, and no signal in negative controls (Fig 3Bii). This evidence concerns the gene PLCD3 and neoplasm.