The electrostatic complementarity between Region 1 and Region 3 have been assumed to lead to an effective head-to-tail interaction between PrP-E200K units and to initiation of the aggregation process, where the protein monomers are still in the cellular folded conformation, PrPC-E200K—the expression “early aggregation” will be used here to denote aggregates of PrPC-E200K units to be distinguished from the amyloid aggregates of disease-associated scrapie proteins, PrPSc-E200K. Here, PRNP is linked to scrapie.