To further clarify whether knockdown of ITGB5 and activation of TIMP1 and TMEM176B expression affect tumor cell invasion, we used dCas9-KRAB-mediated CRISPRi to knock down ITGB5 expression, and used dCas9-VPR-mediated CRISPRa to activate TIMP1 and TMEM176B expression. The gene discussed is ITGB5; the disease is neoplasm.